ABSTRACT
The present study investigated the chemical constituents in the aerial part of Cannabis sativa. The chemical constituents were isolated and purified by silica gel column chromatography and HPLC and identified according to their spectral data and physicochemical properties. Thirteen compounds were isolated from the acetic ether extract of C. sativa and identified as 3',5',4″,2-tetrahydroxy-4'-methoxy-3-methyl-3″-butenyl p-disubstituted benzene ethane(1), 16R-hydroxyoctadeca-9Z,12Z,14E-trienoic acid methyl ester(2),(1'R,2'R)-2'-(2-hydroxypropan-2-yl)-5'-methyl-4-pentyl-1',2',3',4'-tetrahydro-(1,1'-biphenyl)-2,6-diol(3), β-sitosteryl-3-O-β-D-glucopyranosyl-6'-O-palmitate(4), 9S,12S,13S-trihydroxy-10-octadecenoate methyl ester(5), benzyloxy-1-O-β-D-glucopyranoside(6), phenylethyl-O-β-D-glucopyranoside(7), 3Z-enol glucoside(8), α-cannabispiranol-4'-O-β-D-glucopyranose(9), 9S,12S,13S-trihydroxyoctadeca-10E,15Z-dienoic acid(10), uracil(11), o-hydroxybenzoic acid(12), and 2'-O-methyladenosine(13). Compound 1 is a new compound, compound 3 is a new natural product, and compounds 2, 4-8, 10, and 13 were isolated from Cannabis plant for the first time.
Subject(s)
Cannabis , Biological Products , Esters , Dihydrostilbenoids , Plant Components, AerialABSTRACT
Objective: To study the chemical constituents from Medicated Leaven (namely Massa Medicata Fermentata in Chinese). Methods: Compounds were isolated and purified from chloroform extract of Medicated Leaven by column chromatograph of silica gel, ODS, Sephadex LH-20, HPLC, and their structure were elucidated on the basis of spectral data and physicochemical property. Results: Eleven chemical compounds were identified as 5-pentacosyl resorcinol (1), 5-heptadecyl resorcinol (2), 5-heneicosyl resorcinol (3), 5-tricosyl resorcinol (4), (9S,10E,12Z)-9-hydroxy-10,12-octadeca-dienoic acid (5), trans-4-hydroxy-2-nonenoic acid (6), 9,10,11-trihydroxy-11(E)-octadecenoic acid (7), (9S,12R,13S)-9,12,13-trihydroxy-10(E)-octadecenoic acid (8), (9S,12S,13S)- 9,12,13-trihydroxy-10(E)-octadecenoic acid (9), (9S,12S,13S)-9,12,13-trihydroxy-10(E)-octadecenoic acid methyl ester (10), and 9,12(Z)-octadecadienoic acid methyl ester (11). Conclusion: All above compounds are all isolated from Massa Medicata Fermentata for the first time, and the chloroform extract of Massa Medicata Fermentata and compound 8 showed inhibitory effect against TNF-α generation of RAW 264.7 induced by LPS in a dose-dependent manner.
ABSTRACT
Oleaginous microorganisms have emerged as potential sources of oils for biodiesel production. To replenish as an alternative to the vegetable oils, higher lipid accumulating strain coupled with process optimization is indispensable. In the present study, response surface methodology (RSM) based central composite design (CCD) was used for optimization of lipid content from oleaginous fungus Aspergillus sp. Maximum lipid yield of 73.07% (w/w) was achieved at 3% (v/v) inoculum volume, pH 5, glucose 1% (w/v), urea 0.5 % (w/v) and incubation time of 5 (days). Biomass (2.08 g/L) having a lipid content of 73.07 % (w/w) with major constituents of hexadecanoic acid methyl ester and 9-Octadecenoic acid methyl ester were obtained. The lipid composition signifies that from the oleaginous microbe are highly encouraging and desirable to be considered as diesel substitute.